Fig 3.

Expression pattern of rf-PPR592 and Rf-PPR592. (A) Examination of floral bud RNA for expression of rf-PPR592 and Rf-PPR592. RT-PCR of floral bud RNA of a CMS plant (S) with primers specific to rf-PPR592, and RT-PCR of floral bud RNA of an RfRf (nontransgenic) fertile plant with primers specific for Rf-PPR592 (R). DNA, positive control for the amplification where the substrate is leaf DNA from a CMS plant; M, mass markers; 0, no template added, negative control. (B) Examination of different tissues for expression of rf-PPR592. RT-PCR of RNA from different tissues of a CMS plant with primers specific to rf-PPR592. DNA, M, and 0 same as in A.