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Cosegregation of the Rf-PPR592 transgene, restoration of fertility, and reduction of PCF. (A) DNA blot hybridized with an npt II transgene-specific probe. Lane 1, P. parodii CMS line 3688; lanes 2-and 3, sterile T1 progeny of transformed P. parodii; lanes 4–9, fertile T1 progeny. (B) Immunoblot of floral bud proteins probed with anti-PCF antibody. Lanes as in A.
