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. 2025 Sep 25;12:1618464. doi: 10.3389/fmed.2025.1618464

Table 2.

Key clinical trials and outcomes of AAV-mediated gene therapies in hemophilia.

Study/trial name Therapy Target Vector/serotype Key findings Adverse events Clinical phase Ref.
Initial FIX Study AAV2-FIX HB AAV2 Increased FIX to ~10%, then declined Transient ALT elevation Pilot study (111)
UCL AAV8-FIX HB AAV8 Sustained FIX expression, improved transduction Reduced immunoresistance (~25%) Preclinical/early clinical (112)
AMT-061/HOPE-B Etranacogene HB AAV5 Mean FIX activity 36.9%, ↓64% bleeding, ↓97% FIX use No corticosteroids needed Phase 3 (113)
SPK-9001 Fidanacogene HB Engineered AAV FIX activity 22.9%, ABR ↓ from 8.9 to 0.4 CpG-induced transaminitis Phase 1/2 (166)
BBM-H901 Codon-optimized FIX HB Modified liver-tropic AAV FIX ~36.9% at 1 yr. ALT/aspartate elevation in 20% Phase 1 (115)
BMN 270 (BioMarin) Codon-optimized FVIII HA AAV (B-domain) Mean FVIII ↑ to 41.9 IU/dL ALT increase Phase 3 (116)
SPK-8011 Codon-optimized FVIII HA Bioengineered LK03 capsid 16/18 maintained FVIII expression ALT-related expression loss in 2 Phase 3 (117)

“↑” Signifies an increase (for instance, in FVIII or FIX activity), whereas “↓” represents a decrease (such as in bleeding rate or factor consumption). The symbol “~” signifies approximately.