Figure 3.

RNA-blot analysis of GA5, GA4, GASA1, GAI, and RGA mRNA accumulation in bri1-201 and WT upon GA₃ treatment. Poly(A⁺) RNA (1 μg lane⁻¹) from 16-d-old plants grown on Murashige and Skoog medium without or with 50 μm GA₃ added for the last 48 h. Ribonucleic ³²P-CTP antisense probes were synthesized using T7 RNA polymerase from partial cDNA 3′ sequences cloned in the pGEM-Teasy vector. GA5, GA4, and GASA1 hybridizations were performed on the same filter, whereas GAI and RGA hybridizations were performed on independent filters. Radioactive signals were quantified on all membranes and standardized (WT con = 100) by comparison to signals obtained after subsequent blotting with the EF1-α probe.