Figure 5.

Effects of BR and GA on GA5-LUC expression. A, LUC imaging of the GA5-LUC reporter in 16-d-old WT-GA5-LUC and cpd-GA5-LUC seedlings treated with 1 μm EBR for various times. Insert in A, GA5 transcript accumulation monitored by RNA-blot analysis from 16-d-old cpd seedlings treated with 1 μm EBR for 2 h. Hybridization conditions and normalization with EF1-α were performed as described in Figure 3. B, One-week-old cpd-GA5-LUC seedlings grown on Murashige and Skoog plates were transferred to liquid one-half-strength Murashige and Skoog in presence or absence of ancymidol (1 mg L⁻¹) and grown for 7 more d. BR (0.1 μm EBR) and GA (50 μm GA₃) treatments were performed for 16 h by adding the hormones to the medium. C, GA5 transcript accumulation in 16-d-old cpd-ga1-1 double mutant seedlings treated with 1 μm EBR for 16 h. Hybridization conditions and normalization with EF1-α were performed as described in Figure 3. In A and B, bioluminescent LUC images (displayed in pseudocolors) acquired by CCD camera are shown below the quantification (average gray values) of LUC images.