Figure 2.

Distribution of chain length of total α-polysaccharides from wild-type, ae, wx, and aeae/wxwx rice endosperm as determined by HPAEC-PAD. Amylopectin was debranched with isoamylase from P. amyloderamosa, reduced with sodium borohydride, and then fractionated on a Carbopac PA1 column. The α-1,4-glucan chains were eluted with a gradient of sodium hydroxide and sodium acetate and monitored with a PAD. A, The distribution of α-1,4-glucan chains in amylopectin from the wild type (AeAe/WxWx) and the ae mutant (aeae/WxWx). B, The difference in amylopectin chain lengths between the ae mutant and the wild type. The columns show the area of each peak for the ae mutant minus the area of the corresponding peak with the same DP for the wild type. C, The distribution of α-1,4-glucan chains in amylopectin from the wx mutant (AeAe/wxwx) and the double-recessive mutant (aeae/wxwx). D, The difference in amylopectin chain lengths between the double mutant and the wx mutant. The results show representatives from three experiments that gave similar results.