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Excystation rates of protocols 1 to 4 over time. In protocols 1 and 3A, the excystation rate increased after 1 hr of incubation, while in protocols 2 and 3B, it increased after 4 hr and 6 hr, respectively. After the time shown in the graph, no further increase was observed in any of the protocols. Data are shown as the mean ± SD.
