Table 2. Excystation protocols 5 to 11.

Protocol 5	0.25% trypsin + 0.1% STC in HBSS at 39°C for 24 hr
Protocol 6	0.25% trypsin + 0.1% STC in HBSS at 40°C for 24 hr
Protocol 7	0.25% trypsin + 0.1% STC in HBSS at 37°C for 1 hr	→	0.75% STC + 2.5 mM MgCl2 in RPMI medium at 37°C for 24 hr
Protocol 8	0.25% trypsin + 0.75% STC in DMEM at 37°C for 1 hr	→	0.75% STC + 2.5 mM MgCl2 in RPMI medium at 37°C for 24 hr
Protocol 9	0.25% pancreatin + 0.1% STC in HBSS at 37°C for 24 hr
Protocol 10	0.25% α-chymotrypsin + 0.1% STC in HBSS at 37°C for 24 hr
Protocol 11	0.25% protease + 0.1% STC in HBSS at 37°C for 24 hr

Protocols 5 to 11 were modified from protocol 1. In all protocols, vortexing with glass beads was conducted to disrupt oocyst walls as the first step of the excystation process. STC: sodium taurocholate.