Table I.
Comparison of the linkage structure of neutral cell wall polysaccharides from epidermis and mesophyll cells of the elongating maize coleoptile
| Sugar and Linkage | Epidermis | Mesophyll |
|---|---|---|
| mol % | ||
| Fuc | ||
| t-Fuc | 0.6 | 0.3 |
| Rhamnose | ||
| 2-Rha | 0.8 | 0.9 |
| 2,4-Rha | 1.9 | 1.0 |
| Ara | ||
| t-Ara | 25.9 | 21.2 |
| 2-Ara | 0.1 | 0.1 |
| 3-Ara | 1.1 | 0.8 |
| 5-Ara | 1.7 | 1.5 |
| 3,5-Ara | 0.4 | 0.3 |
| Xyl | ||
| t-Xyl | 3.2 | 4.9 |
| 2-Xyl | 1.1 | 1.2 |
| 4-Xyl | 4.4 | 4.5 |
| 3,4-Xyl | 27.7 | 20.6 |
| Man | ||
| t-Man | tr | tr |
| 4-Man | 2.6 | 1.2 |
| 4,6-Man | 0.4 | 0.2 |
| Gal | ||
| t-Gal | 3.9 | 3.3 |
| 3-Gal | tr | 0.6 |
| 4-Gal | 1.4 | 2.8 |
| 6-Gal | 0.5 | 0.8 |
| 3,6-Gal | 1.1 | 0.2 |
| Glc | ||
| t-Glc | 0.1 | 0.2 |
| 3-Glc | 3.1 | 4.8 |
| 4-Glc | 14.9 | 23.9 |
| 4,6-Glc | 2.4 | 3.4 |
Linkage refers to the hydroxyl position upon which another sugar is attached and is inferred from methylation analysis. For example, 4-Glc is itself attached to another sugar via its anomeric carbon (C-1), which is understood, and another sugar was attached at its O-4 position. The actual derivative is 2,3,6-tri-O-methyl-(1-deuterio)-1,4,5-tri-O-acetylglucitol. t-Glc is a nonreducing end-terminal glucosyl residue. tr, Trace.