Table III.
Soluble and cell wall monosaccharide distributions after digestion or extraction of epidermal peels of the elongating maize coleoptile
| Sample | Monosaccharide
|
||||||
|---|---|---|---|---|---|---|---|
| Fuc | Rha | Ara | Xyl | Man | Gal | Glc | |
| mol % | |||||||
| Soluble fraction | |||||||
| NaClO2 soluble [23] | nd | tr | 24.1 | 28.5 | 15.1 | 3.8 | 28.7 |
| NaOH soluble [67] | tr | 1.1 | 29.4 | 36.4 | 10.6 | 4.0 | 18.3 |
| NaClO2+ NaOH soluble [217] | 0.3 | 1.2 | 35.6 | 42.1 | 4.0 | 5.2 | 11.6 |
| Endoglucanase soluble [66] | nd | nd | 1.5 | 1.3 | 2.0 | 5.8 | 89.5 |
| [− NaClO2 − NaOH]- endo soluble [54] | nd | 0.7 | 9.8 | 10.8 | 0.3 | 5.3 | 73.2 |
| Insoluble remainder | |||||||
| Total wall | 0.5 | 2.7 | 29.0 | 37.5 | 3.0 | 6.9 | 20.4 |
| − 20 mM CDTA | 0.4 | 2.1 | 31.0 | 39.9 | 2.5 | 5.9 | 18.1 |
| − CDTA − endoglucanase | tr | 1.5 | 33.8 | 45.6 | 2.6 | 6.5 | 10.0 |
| − 0.34 M NaClO2 | 0.3 | 3.2 | 30.2 | 39.2 | 2.7 | 5.4 | 18.8 |
| − 0.1 M NaOH | 0.3 | 1.6 | 27.7 | 37.7 | 2.8 | 6.5 | 23.3 |
| − NaClO2 − NaOH | 0.2 | 1.9 | 23.8 | 35.0 | 3.0 | 7.0 | 29.1 |
| − NaClO2 − NaOH − endoglucanase | 0.3 | 2.1 | 23.9 | 40.7 | 3.9 | 8.7 | 20.4 |
tr, Trace amounts below 0.2%; nd, not detected. Insoluble remainder represents the non-cellulosic cell wall monosaccharides remaining after enzymic digestion and/or chemical extraction. Soluble fractions from chemical extractions were neutralized and dialyzed extensively with water before freeze drying. Values in brackets are the yields of material in micrograms per milligram of epidermal wall (about 400 μg total non-cellulosic material per mg). Endoglucanase soluble represents monosaccharide released from enzymic digestion of the total wall, whereas [− NaClO2 − NaOH]- endo soluble represents monosaccharide released from insoluble wall material remaining after chlorite and NaOH extraction.