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RNA gel-blot analysis of the expression of two lupin APases in developing P-sufficient and P-deficient root tissues of lupin. Each lane contains 15 μg of total RNA from normal (N) or proteoid (P) root tissue under +P (+) or −P (−) conditions. Samples were collected from 8 to 14 DAE. The blots were hybridized with inserts encoding either the sAPase (sAP) or mAPase (mAP). Note that emerged proteoid roots are not present on plants 8 DAE, so whole roots are collected.
