Table II.
Characteristics of HCR mutants
| Mutants | High CO2Requirementa
|
|||||
|---|---|---|---|---|---|---|
| Air | Low CO2 | Arg7 Insertb | Vector Sequence Present | Cosegregation with Arg+ Phenotype and Arg7 Insert | Diploid Analysis | |
| cia-5 | Leakyc | Stringentc | NAd | NA | NA | Recessive |
| HCRP34 | Leaky | Stringent | 1 | +e | Yes | Recessive |
| HCR209 | Leaky | Stringent | 2 | + | NDf | Recessive |
| HCR3510 | Wild type | Stringent | 1 | + | Yes | Recessive |
| HCR86 | Stringent | Stringent | 1 | + | Yes | Recessive |
| HCR89 | Leaky | Leaky | 1 | − | Yes | Recessive |
| HCR90 | Leaky | Stringent | 1 | + | Yes | Recessive |
| HCR95 | Leaky | Leaky | 1 | + | No | Recessive |
| HCR105 | Stringent | Stringent | 1 | ND | No | Recessive |
a
Growth phenotype in low CO2 was determined by spot test on agar in high CO2 (5% [v/v] CO2 in air) versus either air (300–500 μL L−1 CO2) or low CO2 (50–100 μL L−1 CO2).
b
Arg7 insert detected by Southern analysis using 1.3-kb SalI fragment of Arg7 as probe.
c
Leaky, Cells grow very slowly; stringent, cells did not grow at all.
d
NA, Not applicable.
e
+/− Indicate the presence (+) or absence (−) of vector sequence.
f
ND, Not determined.