Table I.
Oxygen evolution, functional manganese, and D1 protein content of wild type and peripheral accessory Ch1 ligand mutants
| Strain | Growth Condition | Oxygen Evolution | Manganese Content (Mn/250 Chl) | D1 Content/Ch1 (Relative Mean Intensity) |
|---|---|---|---|---|
| μmol O2 mg Chl h−1 | ||||
| WT | Light | 225.7 ± 1.6 | 4.02 ± 0.10 | 100 |
| WT | Dark | 0 | 0 | 88 ± 3.8 |
| D1-H118Q | Light | 134 ± 5.6 (59) | 4.0 ± 0.1 (100) | ND |
| D2-H117N | Light | 105 ± 1.1 (47) | 2.54 ± 0.10 (63) | 85 ± 9.9 |
| D2-H117N | Dark | 0 | 0 | 84 ± 6.4 |
| D2-H117Q | Light | 125 ± 1.1 (55) | 2.11 ± 0.13 (52) | 88 ± 5.0 |
| D2-H117Q | Dark | 0 | 0 | 106 ± 12.5 |
Oxygen evolution was determined at sub-saturating light intensities using thylakoids. Functional manganese content was measured in PSII particles. D1 protein content was determined using thylakoids. Light intensity was 800 umol photons m2 s−1 (67% saturating light intensity for wild-type thylakoids). Data presented as mean ± se. Values in parentheses are percentages of wild type. ND, Not determined; WT, wild type.