Table IV.

Estimation of subcellular metabolite concentrations in developing potato tubers

Metabolite	Total Tissue Content	Amyloplast	Cytosol	Vacuole
	nmol g fresh wt−1	mm
Citratea	18,860	5.6	2.1	31.1
Fumaratea	200	0.059	0.022	0.329
Isocitratea	170	0.050	0.019	0.280
Malatea	5,390	1.6	0.6	8.9
Quinatea	15,670	4.6	1.7	25.8
Shikimatea	370	0.48	0.27	0.48
Succinatea	970	1.26	1.27	1.27
Frub	900	1.11	n.d.	1.32
Glca	23,840	16	31	32
Mana	140	0.04	0.02	0.23
Suca	25,910	10	41	35
Fru-6-Pb	40	0.19	0.14	0.001
Glc-6-Pa	210	0.97	0.74	0.01
Inositola	60	0.078	0.044	0.078
Mannitola	60	0.078	0.044	0.078
Alaa	1,680	2.2	1.2	2.2
Aspa	1,270	4.6	3.7	0.5
Glub	1,800	6.5	5.2	0.7
Glya	230	0.30	0.17	0.30
Iso-Leua	940	0.94	1.71	1.09
Leua	430	0.41	1.37	0.39
Lysa	1,060	0.31	0.12	1.75
Meta	650	0.63	0.19	0.95
Phea	590	0.77	0.43	0.77
Proa	180	0.16	0.63	0.15
Sera	1,340	1.7	1.0	1.7
Thrb	1,100	1.4	0.8	1.4
Tyrb	900	1.17	0.66	1.17
Vala	2,510	3.3	1.8	3.3

Calculations were done using data from Table II and the estimated subcellular volumes shown in Figure 3. n.d., Not detected.

^a Calculations were done using the measurements of the total tissue content from Roessner et al. (2000). 

^b Calculations were done using the measurements of the total tissue content from Trethewey et al. (1998).