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. 2025 Sep 26;16:1618677. doi: 10.3389/fimmu.2025.1618677

Figure 3.

Panel A shows a volcano plot with points colored red (upregulated), blue (downregulated), and black (not significantly changed). Notable genes IL23R, RORC, and FOXP3 are highlighted. Panel B displays a network diagram of gene interactions, with a zoomed-in section highlighting connections among IL23R, RORC, and FOXP3. Green dashed lines indicate fold-change thresholds.

Gene profiling of RNA-seq and PPI analysis reveals direct interaction between Rorc and Foxp3 in HuR-KO Tregs in Foxp3 YFP/Cre HuR fl/fl Tregs. (A) Volcano plot visualization of RNA-Seq results from sorted YFP+ (HuR-KO Tregs) isolated from the spleen of Foxp3 YFP/Cre HuR fl/fl mice. The plot shows the expression levels of 2220 statistically significant genes in YFP+ (HuR-KO Tregs), with differentially expressed genes highlighted (upregulated in red, downregulated in blue). FOXP3, Rorc, and IL-23R, were identified and labeled. (B) Protein-protein interaction (PPI) network analysis using Cytoscape identified significant gene interactions. Among the differentially expressed genes in YFP+ (HuR-KO Tregs), Rorc (RORγt) was found to directly interact with FOXP3, as shown in the enlarged region (red dashed box). n = 6 mice.