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. 2025 Sep 26;16:1618677. doi: 10.3389/fimmu.2025.1618677

Figure 4.

Bar graphs A to F compare mRNA fold changes in WT Tregs and HuR-KO Tregs for six genes: A) Rorc, B) Il23r, C) Ctla4, D) Il2r, E) Il10, and F) Il17a. Significant differences are marked with asterisks, showing decreased expression in HuR-KO Tregs for A, B, C, and F, while E shows increased expression. D shows a trend toward decreased expression with P = 0.054.

Altered mRNA expression of selected genes involved in T helper differentiation and Treg function in Foxp3 YFP/Cre HuR fl/fl Tregs compared to WT control Tregs. (A-F) mRNA expression of key genes involved in T helper differentiation (based on RNA-seq IPA analysis) and Treg function including Rorc, Il23r, Ctla4, Il2r, Il10 and Il17a was measured by qPCR in CD4+CD25+ Tregs isolated from the spleens of HuR-KO and WT control mice, following 4 days of activation with anti-CD3 and anti-CD28. Data were analyzed using unpaired Student’s t-test. Results are representative of four independent HuR-KO mice and a total of six corresponding WT controls, from cohorts distinct from those used in RNA-seq analysis.