Fig. 2.

OsDUF846.2 expression pattern analysis. (A) GUS staining results of different tissue sites for stem, leaf, sheath, ligule, stem internode, glume, and anther. (B) Relative expression levels of different tissue sites. (C) GUS staining of roots of proOsDUF846.2::GUS transgenic plants under abiotic stresses. Student’s t-test was used to calculate the P values (n = 3), *P < 0.05, and **P < 0.01. The error bars represent the mean ± standard error (n = 3). Scale bars: 2 mm. (D) The expression levels of the OsDUF846.2 gene during 24 h of treatment with 120mmol/L and 180 mmol/L NaCl, 20% PEG, 4℃, 38℃, and 42℃, respectively. The error bars represent the mean ± standard error, One-way ANOVA test was used to calculate the P values (n = 3), *P < 0.05, and **P < 0.01