FIG. 4.

Compound 1 does not induce the breakdown of type III secreted proteins. To determine if compound 1 affects the stability of the type III secreted proteins or the autotransporter EspC, bacterial supernatant containing secreted proteins from wild-type EPEC was incubated with 40 μM compound. (A) The amount of EspB was determined by ELISA. The results are shown ± standard deviation and are the average of three different experiments performed in triplicate. (B) The amounts of Tir and EspB were determined by Western blot analysis, and the amount of EspC was monitored by Sypro-Ruby protein stain (Bio-Rad).