FIG. 6.

Virulence-associated promoters are more sensitive to transcriptional inhibition by compound 1. Wild-type EPEC cells carrying the indicated transcriptional fusions (Table 1) were grown in DMEM at 37°C in 5% CO₂ for 8 h with the indicated amount of compound 1 (C1) or DMSO. Chloramphenicol acetyltransferase (CAT) microplate assays and protein determinations to calculate specific activity were performed and graphed as a percentage of CAT specific activity compared to that in untreated EPEC for each transcriptional fusion. The results are shown ± standard deviation and are the average of at least three different experiments performed in duplicate.