FIG. 7.

Compound 1 does not affect the motility or the level of flagellin of EPEC. (A) Strains of bacteria were stabbed into motility agar containing 40 μM compound 1 (+ 1) or compound 2 (+ 2) and grown for 18 h at 37°C. The positive control is a motile strain of EAEC, and the negative control is a nonmotile strain of EAEC. (B) Wild-type EPEC, the ΔescN type III mutant, or the ΔfliC flagellin filament subunit mutant were grown in DMEM for 5 h with 40 μM compound or DMSO as indicated. The bacteria were harvested by centrifugation and lysed in SDS sample buffer to yield the bacterial pellet, and the culture supernatant yielded the secreted proteins. The level of flagellin in the samples was determined by Western blot analysis.