TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| EPEC E2348/69 | Prototypic O127:H6 EPEC strain | 8 |
| ΔescN | escN deleted from E2348/69 | 13 |
| ΔescC | escC deleted from E2348/69 | 13 |
| ΔescJ | escJ deleted from E2348/69 | N. Thomas, unpublished |
| EAEC 17-2 | Motile | T. Steiner |
| EAEC 17-2 | Nonmotile | T. Steiner |
| Plasmids | ||
| pescC-HSV | escC with HSV on C-terminal portion cloned into tet gene (BamHI-SalI) of pACYC184 | 13 |
| pKK232-8 | pBR322 derivative containing promoterless chloramphenicol acetyltransferase (cat) gene | Pharmacia |
| pLER-1179 | pKK232-8 derivative containing ler-cat transcriptional fusion | V. H. Bustamante, unpublished |
| pSEPZ-11 | pKK232-8 derivative containing LEE2-cat transcriptional fusion (pLEE2) | 1 |
| pSEPZ-17 | pKK232-8 derivative containing LEE2-cat transcriptional fusion (pLEE2-17) | 1 |
| pTIR394 | pKK232-8 derivative containing tir-cat transcriptional fusion | 37 |
| pLEE4-cat | pKK232-8 derivative containing LEE4-cat transcriptional fusion | Y. Martínez, unpublished |
| pEspC-cat | pKK232-8 derivative containing espC-cat transcriptional fusion | V. I. Martínez, unpublished |
| pCAT232 | pKK232-8 derivative containing bfpA-cat transcriptional fusion | 36 |
| pKptsH-cat | pKK232-8 derivative containing ptsH-cat transcriptional fusion | N. Olivares, unpublished |
| pKaroG-cat | pKK232-8 derivative containing aroG-cat transcriptional fusion | N. Olivares, unpublished |
| pKpyrC-cat | pKK232-8 derivative containing pyrC-cat transcriptional fusion | N. Olivares, unpublished |