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Fitness burden due to MetS mutations. Wild-type cells were grown in competition cocultures with SP-1A2 cells (MetS with A247E) (A) or with SR3 cells (MetS with G54S) (B). Diluted samples were plated on blood agar to enumerate total cells or on selective agar containing REP8839 to enumerate MetS mutant cells. Increased ratios of wild-type cells to MetS mutant cells in the mixed cultures illustrate the varying cost of fitness (C).
