FIG. 4.

Survival of HCV subgenomic-replicon cells after a 5-day treatment with drugs. Ava5 cells were treated with 0.5 mg/ml of SSG, 20 IU/ml of IFN-α, or the combination of both. After 5 days of drug treatment, the compounds were withdrawn and 500 μg/ml of G418 was added to enrich the remaining HCV replicon-positive cells that are capable of growing in the presence of G418. The cells were stained with crystal violet after incubation for another 18, 24, and 30 days in the presence of G418.