TABLE 2.
Primers used in these experiments
| Name | Sequence | Purpose |
|---|---|---|
| 101 | 5′-TGAACCCTTATAAAAGCGAATACAGCTAGG-3′ | Amplification of joint region from circularized Tn5386 and of left junction of Tn5386 |
| 102 | 5′-GCTAAACTGACATTTAAGAAGTTATGAAGAGATAAGTGG-3′ | Amplification of joint region from circularized Tn5386 and of right junction of Tn5386 |
| 103 | 5′-CTGTTATGCGTATTCAGGAATTCG-3′ | Amplification of left Tn5386 junction in D344R and of regenerated Tn5386 target sequence after excision |
| 104 | 5′-GATCATTGCATAAGCGATACTTG-3′ | Amplification of right junction of Tn5386 in D344R and D344SRF and of regenerated target sequence of D344R after Tn5386 excision |
| 105 | 5′-GAAGCTATCAATATTCAGAAGCAATACC-3′ | Amplification of integrase and excisase of Tn5386 and of right junctions in D344R and D344SRF |
| 201 | 5′-GTTTTGACCTTGATAAAGTGTGATAAGTCC-3′ | Amplification of joint region of Tn916 from D344R |
| 202 | 5′-CACTTCTGACAGCTAAGACATGAG-3′ | Amplification of joint region of Tn916 from D344R |
| 418-1 | 5′-AACAGATGGTGATCGAGAAG-3′ | Creation of probe flanking Tn5386 in D344R and D344SRFa |
| 418-2 | 5′-GGAATCCGTTCTTGGATAGC-3′ | Creation of probe flanking Tn5386 in D344R and D344SRFa |
| 837-RV-1 | 5′-GCTAAGATCAGCACAGCAG-3′ | Creation of probe flanking Tn916 in D344Rb |
| 822rev-1 | 5′-TACGTTGTTTTGCGATATC-3′ | Creation of probe flanking Tn916 in D344Rb |
| 939 R | 5′-TTGCAAATGTCAGCAAGGCTTC-3′ | Amplification of Tn5386 integrase message |
| 735 F | 5′-TATCCGCATGAGCGAAAACG-3′ | Amplification of Tn5386 integrase message |
| 916F | 5′-CCAAAAGTGGCGAACGTCAA-3′ | Amplification of Tn916 integrase message |
| 916R | 5′-AAGACCTTTCATCATGCCGTTG-3′ | Amplification of Tn916 integrase message |