FIG. 4.
Biofilm phenotype of a Salmonella serovar Typhimurium ΔadrA mutant expressing HmsT. The pattern (A), quantification (B), and structure (C) of adherence of Salmonella serovar Typhimurium MAE103 (adrA csgBA) to glass are shown. Growth was in M9 minimal medium at 37°C with shaking for 24 h. Note the thick fibrous biofilm produced by Salmonella serovar Typhimurium MAE103 complemented with HmsT on a multiple-copy-number plasmid (panel C, lane 5), which leads to enhanced crystal violet binding comparable to that of the positive control (panel B, lane 1). Lanes: 1, Salmonella serovar Typhimurium MAE52 (pBAD30), positive control; 2, Salmonella serovar Typhimurium MAE103 (pLC8.2); 3. Salmonella serovar Typhimurium MAE190 (pAHMS16.3; HmsT+); 4, Salmonella serovar Typhimurium MAE103 (pAHMS16.3; HmsT+); 5, Salmonella serovar Typhimurium MAE103 (pAHMS16.1; HmsT+). Values are averages from two independent experiments, with error bars indicating standard deviations.