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Detection of c-di-GMP. MALDI-TOF analysis of relevant HPLC fractions run in the negative-ion detection mode. c-di-GMP is detected at a mass-to-charge ratio (m/z) of 689 [M-H]. Fractions were derived from Salmonella serovar Typhimurium MAE103 (pLC8.2) (control) (A) and Salmonella serovar Typhimurium MAE103 (pAHMS16, HmsT+) (B). r.i., relative intensity.
