TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Salmonella serovar Typhimurium | ||
| MAE52 | PagfD101; rdar28/37 | 36 |
| MAE 103 | ΔcsgBA102 adrA101::MudJ; white | 36 |
| MAE 190 | ΔcsgBA102 bcsA101::MudJ; white | 45 |
| Y. pestisa | ||
| KIM6+ | Pgm+ Hms+ Pla+ | 11 |
| KIM6-2051+ | Kmr Hms− (hmsT2051::mini-kan) Pla+ | 23 |
| Plasmids | ||
| pAHMS14 | 6.9 kb, Tcr, hmsT+; 3.3-kb EcoRI-PstI fragment from pAHMS8 ligated into pBR322 | 23 |
| pAHMS16 | 4.4 kb, Apr, hmsT+; 1.9-kb kpnI-FspI fragment from pAHMS14 ligated into pUC18 | 23 |
| pAHMS16.1 | 5.6 kb, Tcr, hmsT+; 1.9-kb KpnI-FspI fragment from pAHMS14 ligated into pBR322 | 23 |
| pAHMS16.3 | 10.1 kb, Apr, hmsT+; EcoRI linkers added to 1.9-kb KpnI-FspI fragment from pAHMS16 and ligated into pLC8.2 | 23 |
| pBAD30 | 4.9 kb, Apr, araC+, araBAD promoter upstream of multiple cloning site | 15 |
| pBADHmsT6xH | 6.1 kb, Apr, arabinose-inducible hmsT with C-terminal His tag; 1.2-kb SmaI-EcoRI PCR product from pAHMS16 ligated into pBAD30 | This study |
| pGFPmut3.1 | 3.3 kb, Apr; GFP expression vector | Clontech |
| pGFPmut3.1cat | 3.5 kb, Cmr; modified GFP expression vector | This study |
| pLC8.2 | 8.2 kb, Apr; single-copy-number cloning vector derived from pLC682 | 23 |
| pWJB30 | 6.0 kb, Apr, arabinose-inducible adrA with C-terminal His tag | 38 |
a
All Y. pestis strains are avirulent due to lack of the low-calcium response plasmid pCD1. Strains with a plus sign possess an intact 102-kb pgm locus containing the genes for the hemin storage (hms) and yersiniabactin (Ybt) iron transport systems: All other Y. pestis strains have either a pgm deletion or a mutation within the pgm locus. Abbreviations: Apr, Cmr, and Tcr, resistance to ampicillin, chloramphenicol, and tetracycline, respectively.