TABLE 2.
Swarm expansion on 0.3% agara
Strain | Genotype | Swarm expansion (μm/min) |
---|---|---|
DK1622 | Wild type | 1.84 ± 0.40 |
VP1066 | rasA | 0.49 ± 0.17 |
VP1145 | rasA ΔpilA | 0.50 ± 0.17 |
VP1146b | rasA aglB1 | 0.07 ± 0.10 |
DK3468 | dsp | 0.48 ± 0.10 |
DK10410 | ΔpilA | 0.37 ± 0.12 |
Mid-exponential-phase cells were spotted on a rich medium (Casitone-yeast extract) containing low-percentage (0.3%) agar. The radius of each spot was measured at regular intervals for 94 hours. Results shown are for threefold replicate experiments. See reference 27 for A− S+ swarm expansion data.
The aglB1 point mutation causes an A-motility defect.