FIG. 1.

Potential CRP sites in the rhaSR-rhaBAD promoter regions. (Top) Schematic representation of the rhaSR-rhaBAD intergenic region. Transcription start sites are shown as bent arrows. RNAP, RhaS, and RhaR proteins are shown at their binding sites, and potential CRP binding sites are shown as open boxes. RhaS and RhaR are shown as dimers with two spheres per monomer to represent the N-terminal domain and C-terminal domain. (Bottom) DNA sequence of the divergent rhaSR and rhaBAD promoter region. The DNA sequences of the potential CRP binding sites are highlighted by white boxes with the down arrows indicating the base pair substitutions in each of the down mutants used in this study. Also, the number of base pair matches out of 10 with the consensus CRP binding site is indicated.