TABLE 4.
Effect of rcsD mutations on rprA-lacZ expression
Strain | Relevant genotype | β-Galactosidase expression (sp act)a |
---|---|---|
DH300 | Wild type | 1.3 ± 0.3 |
DH351 | rcsD541 | 4.9 ± 0.6 |
DH354 | rfa-1 | 9.2 ± 0.4 |
DH358 | rfa-1 rcsD541 | 6.4 ± 0.4 |
DH366 | rcsC137 | 37 ± 2.6 |
DH369 | rcsC137 rcsD543 | 3.1 ± 0.5 |
DH371 | rcsC137 rcsD543 | 1.9 ± 0.1 |
DH371/pBAD-rcsD+ | rcsC137 rcsD543/pBAD-rcsD+ | 25 ± 3.0 (without arabinose) |
18.5 ± 3.7 (with arabinose) |
Average of at least two independent kinetic β-galactosidase experiments.