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Effect of the ΔdltA mutation on additional GAS phenotypes. (A) β-Hemolytic (streptolysin S) phenotype on sheep's blood agar. (B) M protein and cysteine proteinase SpeB expression by dot blot analysis. (C) Rate of water-induced autolysis activity as determined by decreasing A600. (D) Phase-contrast transmission microscopy of WT and ΔdltA mutant GAS in exponential and stationary growth phases. Note the long chains formed by the ΔdltA mutant in stationary phase.
