TABLE 1.
Phages, strains, and plasmids used in this work
| Phage, strain, or plasmid | Genotype/features | Source or reference |
|---|---|---|
| Phages | ||
| T4 wt | T4D | I. Molineux |
| T4rI | rI48, single-base deletion at position 195; N66-E97 replaced with MTRALLILNV | D. Hall |
| λkanΔ(SR) | λb515 b519 att::Tn903 cI857 nin5 Δ(SR) | 28 |
| λkan this | λb515 b519 att::Tn903 cI857 nin5 ΔS::this | This study |
| Strains | ||
| CQ21 | E. coli K-12 ara leu lacIq1purE gal his argG rpsL xyl mtl ilv | 28 |
| CQ21λkanΔ(SR) | Lysogen carrying λkanΔ(SR) prophage | 28 |
| CQ21[λkanΔ(SR)] recA srl::Tn10 | Lysogen carrying λkanΔ(SR) prophage | This study |
| CQ21(λkan this) | Lysogen carrying λkan this prophage | This study |
| MG1655 | F−ilvG rfb50 rph1 | E. coli Genetic Stock Center |
| MDS12 | MG1655 with 12 deletions, totalling 376,180 nt, including cryptic prophages | 19 |
| MDS12 tonA::Tn10 lacIq1 | This study | |
| XL-1Blue | E. coli K-12 recA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacZΔM15::Tn10] | Stratagene |
| BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) | Novagen |
| BL21(DE3) tonA::Tn5 slyD::Tetr | This study | |
| Plasmids | ||
| pT4T | pBR322 derivative carrying late promoter and lysis cassette of λ with S gene replaced by T4 t | I. N. Wang |
| pT4This | pT4T with HHHHHHGG inserted between residues 132 and 133 of T | This study |
| pT4TRI | pT4T with rI gene inserted distal to Rz1 gene | This study |
| pZA32-luc | p15A origin, PLlacO-1 promoter, Camr | 25 |
| pZA-RI | pZA32Δluc::rI | This study |
| pZA-RICTD | pZA32Δluc::rICTD, codons 25 to 97 of rI | This study |
| pZA-PhoA | pZA32Δluc::phoA, codons 27-471 of phoA, encoding mature PhoA | This study |
| pZA-ssPhoAΦRICTD | Codons 1-24 of rI in pZA-RI replaced by codons 1-26 of phoA, encoding signal sequence | This study |
| pZA-ssPhoAΦRICTDcmyc | pZA-ssPhoAΦRICTD with 10 codons encoding QKLISEEDL inserted after codon 97 of rI | This study |
| pZA-RITMDΦPhoA | First 24 codons of rI inserted in front of codon 27 of phoA in pZA-PhoA | This study |
| pZA-T | pZA32Δluc::t | This study |
| pZA-TCTD | pZA32Δluc::tCTD, codons 56-218 of t | This study |
| pZA-ssPhoAΦTCTD | Codons 1-55 of t in pZA-T replaced by codons 1-26 codons of phoA, encoding signal sequence | This study |
| pZA-ssPhoAΦTCTDT75I | pZA-ssPhoAΦTCTD carrying T75I rV mutation | This study |
| pZA-TTMDΦPhoA | First 70 codons of t inserted before codon 27 of phoA in pZA-PhoA | This study |
| pZA-T70-218 | pZA32Δluc::t70-218, codons 70-218 of t | This study |
| pZA-FtsITMDΦ70T | Codons 24 to 40 of ftsI, encoding TMD, inserted before codon 70 of t in pZA-T70-218 | This study |
| pZE12-luc | ColE origin, PLlacO-1 promoter, Ampr | 25 |
| pZE12-RI | pZE12 Δluc::rI | This study |
| pZE12-RIcmyc | pZE12 Δluc::rIcmyc with codons encoding QKLISEEDL inserted after codon 97 of rI | This study |
| pZE12-GFPΦRIcmyc | DNA fragment encoding GFP inserted between codons 1 and 2 of rIcmyc in pZE12-luc | This study |
| pDS439 | pBR322 origin, ParaBAD promoter, Ampr, carrying gfpmut2 | 33 |
| pET11a-TCTDhis | pBR322 origin, T7 promoter, Ampr, carrying DNA fragment encoding Met residue, then residues 70-218 of T with HHHHHHGG insertion between residues 132 and 133 | This study |
| pER-t | Carrying lysis cassette of λ except S is replaced by T4 t gene, Ampr, Kanr | 30 |
| pPRI | pBR322 origin, λ pPR′ promoter, Ampr, carrying DNA fragment encoding RI | This study |
| pPRIhis | pPRI with 8 codons encoding GGHHHHHH inserted after codon 97 of rI | This study |
| pET11a-RICTDhis | pBR322 origin, T7 promoter, Ampr, carrying DNA fragment carrying Met codon, then codons 25-97 of RI, followed by codons for sequence GGHHHHHH | This study |