TABLE 2.
Sequences of the oligonucleotides used in this study
| Oligonucleotide | Sequence |
|---|---|
| HindIIIpR′for | CATTAAAGCTTGAAGGAAATA |
| CRzNRIrev | TGTATTTACTTTGTGCCGATGATGGGCAACTCTATCTGCACT |
| CRzNRIfor | AGTGCAGATAGAGTTGCCCATCATCGGCACAAAGTAAATACA |
| ClaIRIrev | ATATATATCGATCTAGACCACTTTGTGAAAAGT |
| 132-H6G2− | GAAAAATCACTTGGAGGACACCACCACCACCACCACGGAGGA |
| 133for | TATCCTGTTGATAAAACT |
| 132-H6G2− | AGTTTTATCAACAGGATATCCTCCGTGGTGGTGGTGGTGGTG |
| 133rev | TCCTCCAAGTGATTTTTC |
| Kpn1RIfor | GCGCGCGGTACCATGGCCTTAAAAGCAACAGCA |
| RIAvrIIrev | ATATATCCTAGGCTCTAGACCACTTTGTGA |
| 25RIKpnfor | ATATATGGTACCAATGTCGATCCTCATTTTGAT |
| ss25RIfor | ATTAAAGAGGAGAAAGGTACCATGAAACAAAGCACTATTGCA |
| ss25RIrev | ATCAAAATGAGGATCGACATTCATTTCTGGTGTCCGGGCTTT |
| KpnItfor | ATATATGGTACCATGGCAGCACCTAGAATA |
| AvrIItrev | ATATATCCTAGGAGCAGCGAACAAATAATTATT |
| 56tKpnIfor | ATATATGGTACCGAGTACTATAAGCAATCAAAG |
| PhoAss70tfor | ATTAAAGAGGAGAAAGGTACCATGAAACAAAGCACTATTGCAC |
| 56tssrev | CTTTAGTTGCTTATAGTACTCCATTTCTGGTGTCCGGGCTTT |
| KpnIphoAfor | ATATATGGTACCCCTGTTCTGGAAAACCGGGCT |
| AvrIIphoArev | ATATATCCTAGGTTATTTCAGCCCCAGGGC |
| RItmphoAfor | ATTAAAGAGGAGAAAGGTACCATGGCCTTAAAAGCAACAGCA |
| RItmphoArev | AGCCCGGTTTTCCAGAACAGGCGCTTCAATCGATGGAGATAA |
| KpnI70tfor | ATATATGGTACCATTGAAAAGGAAAGAACTGCA |
| FtsITM70tfor | ATTAAAGAGGAGAAAGGTACCATGTTTGCGTTGTTATGCGGCTGT |
| FtsITM70trev | TGCAGTTCT TTCCTTTTCAATTCCGAGCAGAAAAGCCAGCGC |
| T4TtmphoAfor | ATTAAAGAGGAGAAAGGTACCATGGCAGCACCTAGAATA TCA |
| T4TtmphoArev | AGCCCGGTTTTCCAGAACAGGAATTTCACTGTATGTTTCATA |
| KpnIgfpfor | ATATATGTTACCATGAGTAAAGGAGAAGAACTT |
| N-RIC-gfprev | AAGTGCTGTTGCTTTTAAGGCTTTTAAGGCTTTGTATAGTTCATCCATGCC |
| C-gfpN-RIfor | GGCATGGATGAACTATACAAAGCCTTAAAAGCAACAGCACTT |
| RI-XbaIrev | GTCGACTCTAGACCACTTTGTGAA |
| NdeI70tfor | ATATATCATATGATTGAAAAGGAAAGAACTGCA |
| BamH1trev | ATATATGGATCCAGCAGCGAACAAATAATTATT |
| 25RINdeIfor | ATATATCATATGAATGTCGATCCTCATTTT |
| RIhisBamrev | ATATATGGATCCTCAGTGGTGGTGGTGGTGGTG |
| RIG2H6for | AACATTAAATTGGAGACTGAAGGAGGACACCACCACCACCACCACTGAAATTCAGCGACTTTTCAC |
| RIG2H6rev | GTGAAAAGTCGCTGAATTTCAGTGGTGGTGGTGGTGGTGTCCTCCTTCAGTCTCCAATTTAATGTT |