FIG. 3.

Detection of intracellular H₂O₂ and ROS in ECaNEp cells infected with M. mycoides subsp. mycoides SC strain Afadé at an MOI of 500 mycoplasmas per cell or treated with H₂O₂. Phase-contrast micrographs (a, c, e, g, and i) and fluorescence micrographs (b, d, f, h, and j) of ECaNEp cells as follows: 20 min after infection with M. mycoides subsp. mycoides SC strain Afadé in the absence of glycerol (a and b); infected with strain Afadé in medium supplemented with glycerol (c and d); infected in medium supplemented with glycerol with strain Afadé pretreated with Fab fragments from anti-GlpO IgG (e and f); pretreated with N-acetyl-l-cysteine and then infected with strain Afadé in medium with glycerol (g and h); incubated for 20 min in medium supplemented with 4.4 mM H₂O₂ (i and j).