FIG. 2.

Northern blot analysis of time course effect of RyhB overproduction on exbBD and sodB mRNAs. fur⁺ and fur cells carrying the pBAD-ryhB plasmid were grown to an optical density at 600 nm of 0.5 and then treated with 0.1% arabinose (ara) to express RyhB. Total RNA was extracted at the indicated time by the hot phenol technique and probed for sodB or exbBD mRNAs with biotinylated oligonucleotides EM33 and EM120, respectively (for probe descriptions, see Materials and Methods). The EM12 probe hybridizes with the loading control 16S rRNA.