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(A) Schematic representation of the trdX-lrp-ftsK region on the C. rodentium chromosome. Short arrows indicate the position of annealing of synthetic oligonucleotides. (B) β-Galactosidase assay performed with the E. coli strains CV975 (ilvIH::lacZ), CV1008 (ilvIH::lacZ lrp::Tn10), and AC13 (CV1008 carrying plasmid pAC12), indicated as lrp+, lrp−, and lrp−/pAC12, respectively. Cells were grown in minimal medium (white bars) and leucine-supplemented minimal medium (gray bars).
