FIG. 2.

β-Glucuronidase assay performed on C. rodentium strains AC49 (ATCC 51459, wild type carrying plasmid pAC47), AC52 (EM2, lrp null carrying plasmid pAC47), AC62 (ATCC 51459, wild type carrying plasmid pAC61), indicated as wild type/pAC47(gusA), lrp⁻/pAC47 (gusA), and wild type/pAC61 (gusA lrp), respectively. Cells were grown in minimal medium (white bars) and leucine-supplemented minimal medium (gray bars). The activity value obtained for strain AC49 grown in the absence of leucine was considered 100% activity. All values are the average of at least three independent experiments.