TABLE 1.

Synthetic oligonucleotides

Gene and Name	Sequence (5′-3′)a	Positions of annealingb
lrp
A	GGGCCGGTCAGGTC	−732/−719
B	CGACACACGGACCTACG	+427/+443
C	TTAGCGCGTCTTAATAACC	+477/+495
D	AGCTGGGATCCGAGG	+773/+787
E	ACCAGAAGTGACGCATCC	+210/+227
F	GTAGGGAATTTACCGGC	−505/−489
G	GGTGAGAAAATGACGATTTG	−373/−354
H	GTGTTATCTGTGTGTCGC	−252/−234
I	GTCAGGCAGGAATAGGG	−41/−25
L	ATGGTAGATAGCAAGAAGCG	+1/+20
N	GCATGCCTTCTTGCTATCTACCAT	+1/+18
O	TGGATTGTAGGGAATTTACCGGC	−512/−489
P	CCGTTGCCTGACGGC	−148/−133
Q	GTGAGTAAACGTCGTTATCTTACC	+1/+24
X	GAAAATGACGATTTGACGCTGTTGGCAATGAATAACTGGgtgtaggctggagctgcttc	+368/−329
Y	TTGCTCTGTTTGACTTCTTCCATAACGACGTAGGTCCGcatatgaatatcctcctta	+432/+469
Z	CCGGTAAAGAAGTACAGGCTATGATGCTGGCCGCCCGGCATGGCgtgtaggctggagctgcttc	+23/+66
W	CGAATATTACGGTGGCCGAGATAATCCTGGATAAGCCGCGTcatatgaatatcctcctta	+457/+497
c1	TTATACGCAAGGCGACAAGG (4)
fim
fimA-rt1	CGCTGACGCCACCTTCA	+516/+532
fimA-rt2	GCCCTGAGTCACTCCCTGTCT	+556/+576
fimB-rt1	CGGGCTACCGCTGGAGAT	+396/+413
fimB-rt2	GTTTGCCAAAGCGAAACCA	+441/+459
fimE-rt1	CGCCGCCTGAAAAATGG	+187/+203
fimE-rt2	AACCGCTTCCCGCTCATC	+232/+249
oNs	AATAAAGAGGAAATATAAATCTGAACAAGTCA	−419/−400
oNa	AACCCCTCACAGGAAGCCAT	−480/−449
sig70s	TCCAGCGTAGAGTCCGAAATC	+259/+279
sig70a	TGCCCATTTCGCGCATAT	+308/+325
A5	GCTGACGCCACCTTCAAAGT	+517/+536
Cs	CAGGAAATGACGGTG	−12/+3
C4	GGTGATTGCCCGTGTTTTTTT	+25/+45
Ds	GCCGCCGGGAACCTATCGCG	+219/+238
D1	CATTGAGGTAGATATCCACGCG	+245/+267
D6	GGTGCAGCTCAGCGTCACCC	+1583/+1602
Da	GGCTGCCGCTCAGATAGA	+1626/+1634
G5	TTGTCCTGAAGTTCGAGTGG	−1/+21
G4	GATGAAATGGTTTCAATCCGG	+318/+338
H3	CGACGTTCACTTGCGGTGTA	+151/+170

^aCapital and lowercase letters indicate bases of lrp or fim DNA, respectively, and of an unpaired tail carrying a restriction site (underlined).

^bPosition of annealing refers to the lrp or fim sequence, with the first base of the translational initiation codon as +1.