TABLE 3.
Real-time PCR analysis of fim gene expression in wild-type and lrp null mutant strainsa
| Gene(s) and medium | Mean expression ± SD
|
|
|---|---|---|
| ATCC 51459 (wild type) | EM2 (lrp) | |
| fimAICDFGH | ||
| Min | 1.00 ± 0.17 | 0.060 ± 0.014 |
| Min + leu | 2.20 ± 0.20 | N.D. |
| fimB | ||
| Min | 1.00 ± 0.20 | 0.90 ± 0.10 |
| Min + leu | 1.20 ± 0.10 | N.D. |
| fimE | ||
| Min | 1.00 ± 0.15 | 1.10 ± 0.26 |
| Min + leu | 1.60 ± 0.22 | N.D. |
a
Cells were grown in minimal medium (Min) or minimal medium plus leucine (Min + leu) and collected at the onset of stationary growth phase. Data are presented as arithmetic mean ± standard deviation. Data indicate relative levels of transcription compared to the wild-type value in minimal growth conditions for each individual transcriptional unit. N.D., not determined.