TABLE 3.
Effects of narL and narP null alleles on on expression from Φ(napFHi-lacZ) and Φ(napFEc-lacZ) constructs
| Strain | Fusion | Genotype
|
LacZ sp acta
|
Activation by:
|
||||
|---|---|---|---|---|---|---|---|---|
| narL | narP | −NO3−−O2 | +NO3−−O2 | +NO2−−O2 | NO3− | NO2− | ||
| VJS4797 | Φ(napFEc-lacZ) Δ85 | + | + | 160 | 1,760 | 3,040 | 11 | 19 |
| VJS5101 | Φ(napFEc-lacZ) Δ85 | − | + | 220 | 8,940 | 7,010 | 40 | 32 |
| VJS5109 | Φ(napFEc-lacZ) Δ85 | + | − | 89 | 140 | 130 | 1.6 | 1.5 |
| VJS5117 | Φ(napFEc-lacZ) Δ85 | − | − | 91 | 88 | 84 | 1.0 | 0.9 |
| VJS6621 | Φ(napFHi-lacZ) Δ110 | + | + | 200 | 2,080 | 770 | 10 | 3.8 |
| VJS6623 | Φ(napFHi-lacZ) Δ110 | − | + | 200 | 1,660 | 1,180 | 8.3 | 5.9 |
| VJS6625 | Φ(napFHi-lacZ) Δ110 | + | − | 190 | 1,640 | 350 | 8.6 | 1.8 |
| VJS6627 | Φ(napFHi-lacZ) Δ110 | − | − | 180 | 180 | 150 | 1.0 | 0.8 |
a
Strains were cultured to the mid-exponential phase in MOPS medium (defined medium with glucose).