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(A and B) SDS-PAGE showing proteins eluted from Ni-NTA agarose after interaction with H-NS alone (A) or a mixture of 6×His-Cnu and H-NS (B). (C) SDS-PAGE of proteins after cross-linking with increasing concentrations of glutaraldehyde (0.00025, 0.005, 0.025, and 0.125%). (D) SDS-PAGE of crude extract of BL21/pETCnu after Cnu overexpression, loading onto a Ni-NTA agarose column, washing of the loaded column, and elution from the column.
