TABLE 2.

Operator sequence change versus SF

Parameter or change from Ori-3	Operator sequence	SF
		CNU	Hha
Position	1 5 1015 202530
Ori-3	5′-CCC-GATC ATTAACTGTGAATGATCGGTGATCCTG - GGG -3′	0.54	0.58
5′ 2-bp deletion	TCATTAACTGTGAATGATCGGTGATCCTG	0.56	0.59
5′ 3-bp deletion	CATTAACTGTGAATGATCGGTGATCCTG	0.50	0.57
5′ 4-bp deletion	ATTAACTGTGAATGATCGGTGATCCTG	0.50	0.51
5′ 5-bp deletion	TTAACTGTGAATGATCGGTGATCCTG	0	0
5′ 10-bp deletion	TGTGAATGATCGGTGATCCTG	0	0
5′ 2-bp addition	AGGAT CATTAACTGTGAATGATCGGTGATCCTG	0.52	0.54
3′ 2-bp deletiona	GAT CATTAACTGTGAATGATCGGTGATCC	0	0
T to A at position 30	GAT CATTAACTGTGAATGATCGGTGATCCAG	0	0
T to C at position 30	GAT CATTAACTGTGAATGATCGGTGATCCCG	0	0
A to G at position 19	GAT CATTAACTGTGAATGGTCGGTGATCCTG	0.49	0.57
3′ 10-bp deletionb	GAT CATTAACTGTGAATGATC	0	0
cnb	ATTAACTGTGAATGATCGGTGATCCT

^aThis deletion results in the same DNA sequence as a change from T to G at position 30 due to the GGG sequence of the vector at the 3′ end of Ori-3.

^bSequence contains only the inverted repeat.