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. 2005 Oct;187(20):7072–7080. doi: 10.1128/JB.187.20.7072-7080.2005

TABLE 1.

Activity of ΔN54SppATk towards various peptidyl-MCA substratesa

Substrateb Sp act (μmol mg protein−1 min−1)
pH 10.0 (%) pH 8.0
Ala-Ala-Phe-MCA 9.80 (100) 4.70
Glt-Ala-Ala-Phe-MCA 2.85 (29) 0.98
Z-Val-Lys-Met-MCA 1.05 (11) 0.05
Phe-MCA 0.15 (1.5) 0.04
Suc-Ala-Ala-Ala-MCA 0.06 (0.6) 0.04
Suc-Ile-Ile-Trp-MCA 0.03 (0.3) n.d.
Suc-Leu-Leu-Val-Tyr-MCA 0.02 (0.2) 0.03
Ala-MCA n.d. 0.04
Suc(OMe)-Ala-Ala-Pro-Val-MCA n.d. 0.01
Suc-Ala-Ala-Pro-Phe-MCA n.d. 0.01
Z-Leu-Leu-Glu-MCA n.d. n.d.
Z-Ala-Ala-Asn-MCA n.d. n.d.
Z-Leu-Leu-Leu-MCA n.d. n.d.
Z-Leu-Arg-Gly-Gly-MCA n.d. n.d.
a

Activities were examined with a substrate concentration of 300 μM in 50 mM CHES (pH 10.0) or 50 mM HEPES (pH 8.0) at 60°C. n.d., activity not detected.

b

Suc, N-succinyl; Suc(OMe), N-methoxysuccinyl.