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. 2005 Oct;187(20):6917–6927. doi: 10.1128/JB.187.20.6917-6927.2005

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FIG. 2. — Detection of ECA_CYC molecules in whole-cell extracts as determined by reverse-phase HPLC. (A) HPLC analysis of a whole-cell extract obtained from strain PR4227 (wild-type). (B) HPLC analysis of a whole-cell extract obtained from strain PR4228 (wecA::Tn10). (C) HPLC analysis of a whole-cell extract obtained from strain PR4227 following treatment of the extract with mild alkali. Details regarding the preparation of whole-cell extracts and the conditions for treatment of the extract with mild alkali are provided in “Materials and Methods.” Peaks 1, 2, 3, 4, and 5 contain ECA_CYC molecules containing zero, one, two, three, and four O-acetyl substituents, respectively. mAU, milliabsorption units.