TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genetic markers or characteristic(s) | Source or referencea |
|---|---|---|
| Strains | ||
| E. coli K-12 | ||
| Sθ864 | F−trp lacZ strA thi upp | 31 |
| Sθ874 | Δ(wzxO16wzxC), P2 eductant of Sθ864 with an extended deletion in the region from udk to his | 31 |
| W3110 | F− λ− IN(rrnD-rrnE) rph-1 | CGSC |
| AB1133 | thr-1 leuB6 Δ(gpt-proA)66 hisG4 argE3 thi-1 rfbd1 lacY1 ara-14 galK2 xyl-5 mtl-1 mgl-51 rpsL31 kdgK51 supE44 | CGSC |
| 21548 | Like AB1133, but wecA::Tn10 | 27 |
| 21568 | Like AB1133, but wecG::Tn10 | 27 |
| 14.5 | λ− F−thr-1 leuB6 tonA31 lacY1 tsx-78 supO eda50 his-4 rfbD1 mgl-51 rpsL136 xyl-5 mtl-1 metF159 thi-1 ara-14 wzxE::cm | P. N. Danese |
| PR4119 | Nonpolar insertion of the gentamicin resistance determinant from pUC19 into the AccI site of the wzyE gene of strain 21548 | This study |
| PR4149 | Like Sθ874, but wecA::Tn10 [21548(P1) × Sθ874] | 35 |
| PR4150 | Like PR4149, but wzxE::cm [14.5(P1) × PR4149] | 35 |
| PR4180 | PR4150/pRL147 (with wild-type wecA under the control of the pBAD promoter) | 35 |
| PR4210 | PR4234/pRL147 (with wild-type wecA under the control of the pBAD promoter) pRL165 (with wild-type wzxE under the control of the lac promoter) | This study |
| PR4214 | PR4237/pRL163 (with wild-type wecG under the control of the pBAD promoter) | This study |
| PR4216 | PR4240/pRL163 (with wild-type wecG under the control of the pBAD promoter) | This study |
| PR4217 | PR4240/pRL164 (with wild-type wzyE-wecG under the control of the pBAD promoter) | This study |
| PR4218 | Like PR4237, but wzzE::km (nonpolar insertion of the aphA-3 kanamycin resistance determinant from pUC18K [31] into wzzE) | This study |
| PR4219 | PR4218/pRL163 (with wild-type wecG under the control of the pBAD promoter) | This study |
| PR4220 | Like W3110, but wzzE::km (nonpolar insertion of the aphA-3 kanamycin resistance determinant from pUC18K [31] into wzzE) | This study |
| PR4221 | PR4220/pCA53 (wild-type wecA-wzzE on a 2.54-kb ClaI fragment cloned in pBR322) | This study |
| PR4222 | Like PR4237, but wzxE::cm [14.5(P1) × PR4237] | This study |
| PR4223 | PR4222/pRL163 (with wild-type wecG under the control of the pBAD promoter) | This study |
| PR4224 | PR4237/pBAD102/D/lacZ | This study |
| PR4225 | PR4234/pACYC184 | This study |
| PR4226 | PR4220/pBR322 | This study |
| PR4227 | W3110/pBR322 | This study |
| PR4228 | Like PR4227, but wecA::Tn10 [21548(P1) × PR4227] | This study |
| PR4233 | PR4234/pRL147 (with wild-type wecA under the control of the pBAD promoter) pRL169 (with wild-type wzxO16 under the control of the lac promoter) | This study |
| PR4234 | Like Sθ874, but wecA::km (nonpolar insertion of the aphA-3 kanamycin resistance determinant from pUC18K [31] into wecA) wzxE::cm [14.5(P1)] | This study |
| PR4236 | PR4234/pRL147 (with wild-type wecA under the control of the pBAD promoter) pRL166 (with wild-type wzxC under the control of the lac promoter) | This study |
| PR4237 | Like W3110, but wecG::Tn10 [21568(P1) × W3110] | This study |
| PR4240 | Like PR4237, but wzyE::gm [PR4119(P1) × PR4237] | This study |
| Salmonella enterica serovar Typhimurium | Wild type | Laboratory collection |
| Shigella dysenteriae | Wild type | ATCC |
| Shigella flexneri | Wild type | ATCC |
| Enterobacter cloacae | Wild type | ATCC |
| Klebsiella pneumoniae | Wild type | ATCC |
| Proteus vulgaris | Wild type | ATCC |
| Pseudomonas aeruginosa | Wild type | ATCC |
| Plasmids | ||
| pBR322 | Cloning vector | Promega |
| pBAD18 | Expression vector | 15 |
| pBAD24 | Expression vector | 15 |
| pBAD102/D-TOPOR | Expression vector | Invitrogen |
| pBAD102/D/lacZ | Expression vector | Invitrogen |
| pACYC184 | Cloning vector | 10 |
| pRL147 | Wild-type wecA under the control of the pBAD promoter in pBAD18 | 35 |
| pRL163 | Wild-type wecG under the control of the pBAD promoter in pBAD102/D-TOPOR | This study |
| pRL164 | Wild-type wzyE-wecG under the control of the pBAD promoter in pBAD102/D-TOPOR | This study |
| pRL165 | Wild-type wzxE under the control of the lac promoter cloned in pACYC184 | This study |
| pRL166 | Wild-type wzxC under the control of the lac promoter cloned in pACYC184 | This study |
| pRL169 | Wild-type wzxO16 under the control of the lac promoter cloned in pACYC184 | This study |
| pCA53 | Wild-type wecA-wzzE on a 2.54-kb ClaI fragment cloned in pBR322 | 26 |
a
CGSC, E. coli Genetic Stock Center and M. Berlyn, Biology Department, Yale University, New Haven, CT; ATCC, American Type Culture Collection, Manassas, VA.