TABLE 2.

MALDI-TOF MS analysis of ECA_CYC molecules purified by reverse-phase HPLC

Peak	Retention time (min)	Molecular ion [M—H]− (Da)		Proposed structure of parent moleculea
		Observedb	Predicted
1	79	2,428.9 ± 1	2,429	[ECACYC]4
2	99	2,471.3 ± 1	2,471	[ECACYC]4 + 1 O-acetyl substituent
3	119	2,515.2 ± 1	2,513	[ECACYC]4 + 2 O-acetyl substituents
4	143	2,555.2 ± 1	2,555	[ECACYC]4 + 3 O-acetyl substituents
5	160	2,597.5 ± 1	2,597	[ECACYC]4 + 4 O-acetyl substituents

^a[ECA_CYC]₄ refers to a molecule of cyclic ECA containing four trisaccharide repeat units.

^bValues are means±standard deviations.