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R. leguminosarum UPM791 hydrogenase gene cluster cloned in plasmid pALPF1. hup and hyp genes are shown by full and empty horizontal arrows, respectively, and designated by capital letters. The locations of characterized promoters in the hydrogenase gene cluster are shown by bent horizontal arrows. DNA fragments deleted in pALPF1 derivative plasmids containing single-gene deletions are shown by horizontal bars.
