FIG. 4.

Subcellular location of HupS and HupL in R. leguminosarum containing a deletion in the hupH gene. Hydrogenase subunits were immunologically detected using antisera generated against HupL (A) and HupS (B). SDS-PAGE gels were loaded with cell membrane (lanes 1, 3, and 5) or soluble (lanes 2, 4, and 6) fractions from microaerobically grown free-living culture cells. Lanes 1 and 2, UPM1155(pALPF1); lanes 3 and 4, UPM1155(pALPF7) (ΔhupH); lanes 5 and 6, SM61(pALPF1). pre and mat are as defined for Fig. 3.