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. 2005 Oct;187(20):7018–7026. doi: 10.1128/JB.187.20.7018-7026.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 7. — Analysis of the formation of a HupS-HupH_Strep complex. Proteins eluted from a Strep-Tactin column loaded with cell extracts from different strains were separated in a 15% SDS-PAGE gel (lanes 2, 3, and 4). Lane 1 contains crude cell extracts from microaerobically grown cells. HupH_Strep (A), HupS (B), and HupL (C) proteins were identified using a streptavidin-alkaline phosphatase conjugate and antisera against HupS and HupL, respectively. Plasmid pPM125 encodes the HupH-Strep-tag fusion. Lanes: 1, UPM1155(pALPF1); 2, UPM1155(pALPF15/pPM125) (ΔhupS/hupGHI); 3, UPM1155(pALPF2/pPM125) (ΔhupL/hupGHI); 4, UPM1155(pALPF4/pPM125) (ΔhupD/hupGHI).