TABLE 2.
Primers used in this work
| Primer | Sequence (5′-3′) | Use |
|---|---|---|
| HUPS5 | ACTGCCGAGACTTTTTATGACGTCATTCGCCGCCAGGGGATTCCGGGGATCCGTCGACC | hupS deletion |
| HUPS3 | GAATCGTCATGATGGTGTTTCCCGCCCAGAATGTTAAGGTGTAGGCTGGAGCTGCTTC | |
| HUPL5 | AAGCGCTTGACCACCAAGCGCGAAAAAGCTGACGCTTAAGTGTAGGCTGGAGCTGCTTC | hupL deletion |
| HUPL3 | TTATCGGACCTGGACCCTGGCCATTTCTTGCCCATCCGGCATATGAATATCCTCCTTAGT | |
| HUPD5 | GATCTCGGGTGAACGGCTCTTCAAGGACAGGGAGGATTAGGTGTAGGCTGGAGCTGCTTC | hupD deletion |
| HUPD3 | GCAGTTCAACGCGGCCGGTTCGGCACGGCGCTCGTAACGCATATGAATATCCTCCTTAGT | |
| HUPG5 | TCGCAATGTTTTGCATCGTGATTTGTGGAGGAGACAATGATTCCGGGGATCCGTCGACC | hupG deletion |
| HUPG3 | TTCATCGCGCGATCTCCTTGCCGGCATGGGTGATCTCGAGTGTAGGCTGGAGCTGCTTC | |
| HUPH5 | CGAGATCACCCATGCCGGCAAGGAGATCGCGCGATGAGTGTAGGCTGGAGCTGCTTC | hupH deletion |
| HUPH3 | CGCTCATTTGAAATAGGCCTCCCAGATGTCCCGCAGTCGATATGAATATCCTCCTTAGT | |
| HUPI5 | CGACTGCGGGACATCTGGGAGGCCTATTTCAAATGAGCGATTCCGGGGATCCGTCGACC | hupI deletion |
| HUPI3 | CCATCGCCAAGCCTCATGAACTTCGACTGCAGGGCATCGGTGTAGGCTGGAGCTGCTTC | |
| HUPJ5 | CTGCAGCTCGAGATGCGCTACCGGGAGATTTATGCGACCATTCCGGGGATCCGTCGACC | hupJ deletion |
| HUPJ3 | CCGAGAAGGAATGTCATTCCGATGCCTCCTCGCGCCCGCGTGTAGGCTGGAGCTGCTTC | |
| HUPH5 | CGAGATCACCCATGCCGGCAAGGAGATCGCGCGATGAGTGTAGGCTGGAGCTGCTTC | pALPF34 |
| TAGH | GGCGCCTTCGCCCTCGGGGGCAACCCAAAAACCAGCCTTCTTCTCGAACTGCGGGTGGC | |
| TAGI | CTCATATGTGGAGC CACCCGCAGTTCGAGAAGAGCGCCTTCGAGAATTT | pPM164 |
| MAN2 | GCGGTCGCATAAATCT | |
| KNDE | GCCACAGTCGTCATATGACATTCCTTCTCGGGGC | pPM165 |
| MANK3 | TGCCGCGCCGTCTCACAG | |
| TAGG | CTCATATGTGGAGCCACCCGCAGTTCGAGAAGCCATCTGCCCTGGTCCG | pPM166 |
| MAN2 | GCGGTCGCATAAATCT | |
| MANF5 | GCGATGCTCGGCTTGCTG | pPM125 |
| MAN2 | GCGGTCGCATAAATCT | |
| STREP1 | GTGTAGGCTGGAGCTGCTTC | pPM70 |
| STREP2 | CTTTTCGAACTGCGGGTGGCTCCAGTTCATATGAATATCCTCC |